Autor: MENEZES, G.B.

Orientador: FRANCISCHI, J.N.

Outros autores: MALTOS, K L M;;

Linhas de pesquisa no CNPq: CIENCIAS BIOLOGICAS / FARMACOLOGIA

Unidade: INSTITUTO DE CIÊNCIAS BIOLÓGICAS
Departamento: FISOLOGIA E FARMACOLOGIA

Palavras-Chave: CELL RECRUITMENT - COX-2 INHIBITORS - NSAIDS

Aim: Analgesic, antipyretic and anti-oedematogenic activities were already atributed to specific-cyclooxygenase (COX)-2 inhibitors. However, data on inhibition of leukocyte recruitment by these drugs are still lacking in the literature. The aim of the present study was to compare the inhibitory potencial of specific COX-2 inhibitors (celecoxib and rofecoxib) with indomethacin and piroxicam, considered unspecific COX inhibitors in a standard model of cell migration. Methods and Results: Female Holtzman rats (140-180g) were pre-treated (T, 30 min before stimuli) subcutaneously with 6mg/Kg celecoxib, 0.7mg/Kg rofecoxib or 2mg/Kg indomethacin and injected intraperitoneally with LPS (E.coli endotoxin, B4 :111, 0.3?g in 1mL/site) or saline (C) in peritoneal cavity. The animals were sacrificed and the lavage fluid collected after 6 hours of LPS injection and prepared for total and specific cell counting. Cell migration was dose-dependently inhibited by celecoxib (T=11.18?1.59 x 103; C=19.75?1.50x103); rofecoxib (T=12.13?2.33 x103; C=20.16 ? 1.43 x 103) and indomethacin (T=12.60?1.07 x103; C=20.06 ? 2.02 x 103). This effect was due a significant reduction on lymphocyte, neutrophil and mast cell numbers. Surprisingly, a more specific COX-1 inhibitor, piroxicam, did not significantly affect cell recruitment due to LPS. Conclusion: Reduction of cell recruitment seems to be related to specific COX-2 inhibition, thus implying this isoform in cell recruitment induced by bacterial endotoxin.

Apoio: CNPq, FAPEMIG e CAPESp>